Pornima Kamble, Mishel Rodrigues, KavitaPandey

Isolation of Gluten Hydrolyzing Probiotic Bacteria
Int.J.Adv.Microbiol.Health.Res.2018; 2(4):10-15

Publisher: IJAMHR, Category: Current Issues

Abstract

Gluten, a common component in the human diet, is capable of triggering coeliac disease pathogenesis in genetically predisposed individuals.Coeliac disease is characterized by intestinal inflammation caused by gluten. Mammalian digestive enzymes are only partly capable of cleaving gluten producing residual fragments which are capable of inducing toxic responses in patients with coeliac diseases.It is a common fact that fermented food like curd is a rich source of probiotics(good bacteria) that are beneficial for the intestinal functioning. The current project was undertaken to isolate gluten hydrolyzing probiotic bacteria from functional food like curd. Gluten used was extracted from wheat dough. Bacterial isolates obtained from curd were cultured and maintained on MRSA agar. Out of the 8 isolates, growth of 6 was supported by 0.5% gluten solution (acting as Carbon as well as Nitrogen source). Thus, the gluten hydrolyzing probiotic strains were further subjected to biochemical tests for identification. The isolates in isolation or combination could be used in treatment of patients suffering from coeliac diseases (CD).